Sperm and eggs are collected, fertilized in the laboratory and the resulting embryos replaced into the uterus. It is performed in couples having blocked tubes, low sperm count, endometriosis, unexplained infertility etc. Testicular biopsy.

The centre has OLYMPUS Inverted Microscope with Narishige micromanipulator for ICSI. ICSI is the injection of single sperm into each vaible egg, the objective being to increase the chance of fertilisation and development of embryo used in conjunction with a IVF procedure. ICSI is designed to help couples where male partner has recognised sperm problem, previously failed IVF cycles, increased age of female partner etc. Retrieval of sperms directly from the testes for use in ICSI.Assisted hatching and Blastocyst Culture for improving success in IVF and ICSI are also done here.
ICSI has had a great impact on male infertility as IVF had on tubal infertility.

Embryo freezing (Trivector) is used for Cryopreservation of excess embryos which can be used in subsequent cycles.

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Kaival Maternity Home and Surgical Hospital is now equipped with revolutionary medical technology from Medison.

Medison 9900 is a latest innovation with color Doppler,3-D and 4-D facilities.

Statistically about 4 to 5 congenital and maternal anomalies are detected from every 100 pregnancies. Most common congenital anomalies are cranio-spinal (head and spine) and cardiac (heart) anomalies. They account for about 20-30 % perinatal mortalities. These anomalies occur sporadically and can not be anticipated. Imaging at right time enables us in taking the right decision at the right time.

High resolution ultrasound with 3-D and 4-D facilities enables us better visualization of normal and abnormal foetal parts. We can find out many congenital anomalies like abnormal development of brain, kidneys and limbs, heart problems, any tumor in neck, chest or abdomen. Parents can also appreciate the foetal parts like face, spine, limbs, fingers and every single organ as if they are actually looking at the foetus. 4-D ultrasound enables us to see real time images of foetal movements like yawning, thumb sucking, swallowing etc.

Along with this, we can have a photograph of your child during intranterine (fetal) period and store it as a memory in C.D / Photo.

A covering layer, or 'shell' surrounds embryos or blastocysts called the Zona Pellucida (ZP). The zona has an important role in fertilisation as it allows only one sperm to penetrate the zona and enter the egg to achieve fertilisation. It also acts to prevent premature implantation in the Fallopian tube and may help prevent the early embryo from being attacked by cells of the immune system.
The embryos have to "hatch" or break out of the zona in order to embed into the endometrium lining the uterine cavity. This occurs about four to five days after embryo transfer when the embryo is at the blastocyst stage. Naturally this takes place by expanding/contracting of the zona until it distorts, allowing the blastocyst to "hatch".
If the zona is not functional, this hatching may not occur. It has been reported that up to 75% of normal embryos never hatch through the protective layering of the zona. Laboratory techniques involved in IVF may result in hardening of the zona. In natural fertilsation there are enzymes present within the fluid in the Fallopian tube, which may "soften" the zona. This does not happen in IVF as the tube is bypassed. The zona may also be thicker following IVF, especially in older ladies. Frozen embryos may also have a hardened zona.

Who is suitable for Laser Assisted Hatching?
At the London Fertility Centre patients who will be considered for laser assisted hatching are:
:: Those patients who have IVF or ICSI who are over 37 years
:: Patients having FER
:: Patients who have had a previous failed IVF or ICSI treatment cycle
:: Patients undergoing IVF/ICSI for the first time, who are considered poor responders because they have required a high dose of gonadotrophins for poor ovarian response
:: Patients who in an earlier IVF cycle have had a low fertilisation rate, for example, less than one third of the embryos achieving fertilsation
:: Patients with three or fewer embryos
:: Patients who request laser assisted hatching and are fully informed of its use and function

Embryo Freezing
Embryo freezing (called cryopreservation) is used in conjunction with in vitro fertilization (IVF). Embryo freezing permits high quality embryos that are not immediately used for IVF to be frozen for the couple’s later use.

In a typical IVF cycle, the female partner undergoes hormonal stimulation with fertility medication to ensure that her ovaries produce enough eggs. In many cases, a surplus of eggs are retrieved and successfully fertilized. Selected embryos that are not immediately returned to the woman’s uterus can be frozen in small vials of liquid nitrogen and safely stored for decades.

The Centre has the facility for Pre Implantation genetic screening Chromosomes 18, 21 &22.

How is PGD Done?
Pre-implantation genetic diagnosis is used during an IVF cycle to identify specific embryos that carry abnormal genes. PGD is normally recommended for couples where one or both of the parents are a known carrier of a genetic disease (i.e. hemophilia or cystic fibrosis).

WHAT IS EMBRYO BIOPSY?
When an embryo reaches 4 to 8 cell stage (on day 2 or day3) of development, one or two of these cells, called “blastomeres,” can be removed from the embryo with the same sort of micromanipulation technique that is used for ICSI, and the embryo will be unharmed, and can go on to develop just as though this one cell were never removed. Every cell of the embryo at the four to eight-cell stage is genetically identical to every other cell, and any group of those cells will develop normally on their own because they have the complete genetic potential to develop into a normal embryo. You are not removing any genetic material that is necessary for the complete and normal expression of that embryo’s genes if you take out a single cell at this stage. You can then subject those one or two cells to genetic analysis, and know thereby the genetic composition of the embryos. These genetic tests can be performed within a day, giving plenty of time to decide which embryos to transfer back to the patient and which embryos not to transfer.

Who is Eligible for PGD?
When PGD was first offered to the public, it was meant to be used only by those couples whose future children were at "high risk" of developing a genetic disorder. However, PGD was originally only offered at a select few infertility clinics worldwide.

Who should be offered PGD:
Women over the age of 35 who have had miscarriages or failed attempts at becoming pregnant through IVF
Women who have experienced recurrent miscarriages
Any couple who has repeatedly been unable to become pregnant through multiple IVF cycles
Any couple where a man’s sperm count is low that it is necessary to perform ICSI

What Genetic Diseases Can PGD Test For?
Numerous diseases and disorders classified as either chromosomal disorders, single gene defects or sex-linked disorders can be tested for through PGD. Specific chromosomes are tested for specific disorders, including:

Chromosome 13: Breast and ovarian cancers, deafness, Wilson Disease
Chromosome 15: Marfan Syndrome, Tay-Sach's Disease
Chromosome 16: Polycystic kidney disease, Alpha thalassemia
Chromosome 17: Charcot-Marie-Tooth Disease
Chromosome 18: Niemann-Pick Disease, pancreatic cancer
Chromosome 21: Down's Syndrome
Chromosome X: Duchenne muscular dystrophy, Turner's Syndrome, Fragile X Syndrome
Chromosome Y: Acute myeloid leukemia

What are the risks?
Embryo biopsy procedures have been used extensively at major IVF clinics throughout the world for PGD of both single gene and chromosomal disorders. Follow up studies have shown that the procedure is safe with no known adverse affects on the embryo's potential to implant and develop normally. Some studies have shown that biopsied embryos may have a significantly better chance of implanting in the uterus than non-biopsied embryos.
Due to the complexity of the genetic tests, it is possible that a conclusive result is not obtained from all biopsied embryos. In this case, the embryos will be left to grow further and if they develop to blastocyst stage they will be frozen.

Other PGD Facts
• Embryo biopsy does not appear to affect embryo development.
• Only embryos that are good quality and adequately developed can be biopsied.
• It may not be possible to obtain a result from every embryo.
• It is not possible to test all chromosomes of the embryo using PCR.
• The accuracy of the testing is high, but not 100%.

Prenatal testing is highly recommended in an ensuing pregnancy.

Embryo freezing (Trivector) is used for Cryopreservation of excess embryos which can be used in subsequent cycles.

The centre has also facilities for endoscopic surgeries, sonography and endocrinological tests. The Akanksha infertility centre can boast of innumerable IUI abd IVF pregnancies. The team working in the centre are friendly, approachable and highly knowledgeable and above all are geniunely committed to helping couples achieve their goal and last but not the least providing outstanding infertility treatment at affordable price The Centre Has The facility for Pre Implantation genetic screening Chromosomes 18, 21 &22.

"Unless you are prepared to keep your sight on tomorrow. You cannot expect to be in touch with today.